Versicolour is our multi-colour fibre-based imaging device, intended for clinical evaluation.

Versicolour is an affordable multi-colour wide-field fluorescence microendoscopy platform, capable of simultaneously detecting several disease targets to be used in patients with lung disease. The device was first implemented in the clinic in 2015 in conjunction with the Smartprobes, making it the world’s first demonstration of three-colour in vivo in situ multiplexed optical imaging.

Imaging in the lung is via a small, medically approved optical fibre. Light emitted from multiple light emitting diodes (LEDs) is collimated and combined before being sent to an infinity-corrected microscope objective. The fluorescence exiting the microscope objective is then focused onto a fast CMOS camera. Both the optical fibre and the CMOS camera are available commercially.

Versicolour has a simple user interface and fluorescence-calibration algorithms to ensure standardised imaging. Further adaptations are being undertaken to enable spectroscopy, voice recordings, and eye tracking user interfaces to allow more flexibility and freedom for the operators.

Our system has been tested in a pre-clinical setting on ex vivo animal lungs and has performed well in repeated challenging experiments, showing the ability to perform multi-colour imaging. We have demonstrated the effectiveness of the instrument by simultaneously imaging neutrophils, macrophages and fungus (Aspergillus fumigatus) in ex vivo human lung tissue, with excellent results.

The Versicolour imaging system, with one of the optical fibres.